Methanol extract had a damaging effect on L. procumbens was chosen for further chemical, in vitro and in vivo studies. procumbens is used by the population in Guatemala for topical applications to treat chronic ulcers caused by leishmaniasis. (Asteraceae), also known as bull’s herb in Guatemala and t’ulum (Maya language) in Yucatan, Mexico, was one of the most active. A number of them has been screened looking for in vitro leishmanicidal activity. Besides the problems already mentioned, reported increasing failures of miltefosine, currently used to treat visceral leishmaniasis, some of the possible causes being parasite resistance, discontinuity of treatment, and reinfection.Ĭurrently, there is an urgent need for new leishmanicidal drugs and it is known that medicinal plants are an important source of new molecules with pharmacological activities several plants have been used as treatment for “chiclero’s ulcer” in Mayan traditional medicine. The second-line drugs, such as amphotericin B and pentamidine, may even be more toxic. Chemotherapy with antimonials is the first choice to treat leishmaniasis however, the treatment is prolonged, expensive and not very effective, and may have severe side effects. In Mexico, the Yucatan peninsula is an endemic area for Leishmania mexicana, which causes localized cutaneous lesions, known popularly as “chiclero’s ulcer”, with an incidence of 5/1,000 inhabitants, although it can also induce more severe forms of the disease such as the diffuse, mucocutaneous, or visceral forms. The disease caused by infection with Leishmania parasites has an important impact worldwide, with up to 200 million new cases per year. sativum est un traitement naturel prometteur pour la leishmaniose cutanée et ses effets curatifs en font un bon candidat pour un possible nouveau phytomédicament. L’augmentation du ratio IgG2a/IgG1 a permis d’indiquer une tendance à augmenter une réponse immunitaire de type Th1 dans les souris traitées avec ce mélange. sativum contrôlait mieux l’infection tout en n’étant pas toxique lors de l’essai de toxicité orale aiguë chez la souris. Nous avons pu montrer que le mélange d’extraits de T. Douze semaines après l’infection, un échantillon de sang a été prélevé par ponction cardiaque et les quantités totales d’immunoglobulines IgG, IgG1, et IgG2 ont été déterminées par ELISA indirect non commercial.
L’épaisseur des coussinets plantaires des souris a été mesurée une fois par semaine. sativum, soit avec les 2 extraits séparés. mexicana et traitées soit avec un mélange de T. L’essai in vivo a été réalisé avec des souris infectées avec L. Les lésions hépatiques et d’autres paramètres de toxicité ont été déterminés au 14ème jour. sativum, comparé à un groupe de contrôle. La toxicité orale aiguë a été testée avec la procédure Up and Down (UDP) sur un groupe de souris saines auxquelles ont été administrés soit des extraits de T. Nous avons testé un mélange de Tridax procumbens, dont l’action directe contre le parasite Leishmania mexicana est connue, et d’ Allium sativum, dont l’effet immunomodulateur est connu, comme une alternative pour traiter la leishmaniose cutanée. sativum extracts is a promising natural treatment for cutaneous leishmaniasis and its healing effects make it a good candidate for a possible new phytomedicine. An increase in the ratio of IgG2a/IgG1 indicated a tendency to raise a Th1-type immune response in mice treated with the mixture. mexicana infection while not being toxic when tested in the acute oral toxicity assay in mice. sativum extracts was better at controlling L.
After the 12-week period of infection, blood samples were obtained by cardiac puncture to determine the total IgG, IgG1 and IgG2a immunoglobulins by a noncommercial indirect ELISA. The thickness of the mice’s footpads was measured weekly. mexicana promastigotes and treated with either a mixture of T. The in vivo assay was performed with mice infected with L. Liver injury and other parameters of toxicity were determined in mice at day 14. sativum extracts and compared with a control group. Acute oral toxicity was tested with the Up-and-Down Procedure (UDP) using a group of healthy mice administered with either T. We tested a mixture of Tridax procumbens, known for its direct action against Leishmania mexicana, and Allium sativum, known for its immunomodulatory effect, as an alternative to treat cutaneous leishmaniasis.
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